Table 1

The MID1 and MID2 deletion constructs used in pEGFP-C2 for cellular co-localisation analysis, co-immunoprecipitation and in pPC86/pDBLeu for interaction analysis with Alpha 4. Terms: RF denotes a C3HC4 RING finger; BB denotes C2H2 B-Boxes; CC denotes a Coiled-coil motif; FNIII denotes a Fibronectin Type III domain and CTD denotes a C-terminal domain (encompassing a SPRY domain).

Construct

Amino Acids deleted

Mid1ΔRF

1–69

Mid1ΔBB

71–213

Mid1ΔCC

214–349

Mid1ΔFNIII

370–473

Mid1ΔCTD

483–667

Mid2ΔRF

1–69

Mid2ΔBB

71–213

Mid2ΔCC

214–349

Mid2ΔFNIII

370–473

Mid2ΔCTD

483–686

BB(Mid1)

1–70 and 214–667

CC(Mid1)

1–213 and 350–667

BBCC(Mid1)

1–70 and 350–667

Short et al. BMC Cell Biology 2002 3:1   doi:10.1186/1471-2121-3-1