Table 2

Effect of H7 on the L-T4-induced DAG and Peth formation in [14]oleic acid pre-labeled hepatocytes.

Lipid

Additions

Control

L-T4

% of control


cpm/1 07 cells


NaOH+ethanol

2170 ± 362

5000 ± 638*

230

[14C]Peth

H7 + NaOH + ethanol

1847 ± 296

2915 ± 44*

158

NaOH

10797 ± 715

16515 ± 1077*

153

[14]DAG

H7+NaOH

7359 ± 746

9051 ± 1290

123


[14C]oleic acid labeled hepatocytes were pre-incubated for 15 min with H7 (50 μM) or NaOH (l00 nM) alone prior to stimulation with L-T4 for 2 min. 1.5% ethanol was added 15 min before L-T4 addition or together with H7 (50 μM) or NaOH (100 nM) for determination of Peth formation in the cells. The data are mean ± S.E. of four separate experiments. * P < 0.05 vs. control.

Kavok et al. BMC Cell Biology 2001 2:5   doi:10.1186/1471-2121-2-5

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