Figure 2.

Coprecipitation of α-actinin, but not talin or vinculin, with β1 integrin in HBE and MCF-7 cells. (A) Integrin β1 was immunoprecipitated (IP) from HBE and MCF-7 cells using the anti-β1 integrin antibody or control IgG. The immunoprecipitates were probed with anti-α-actinin, anti-talin, or anti-vinculin antibody. Molecular size markers are indicated at right in kDa. (B) Equal amounts (5 μg protein) of whole cell lysates from quiescent HBE and MCF-7 cells were probed with anti-α-actinin, anti-talin, or anti-vinculin antibody. Molecular size markers are indicated at right in kDa.