Table 1

Changes of intracellular Ca2+ concentration in the cell population with elevated [Ca2+]i, assessed by flow cytometry using Fluo-3fluorescence (arbitrary units) in control and Scott cells after 48 or 72 h of apoptosis induction by calcium ionophore A23187 (200 nM) or/and SKF 96365 (10 μM), in the absence and presence of the caspase inhibitor Ac-DEVD-CHO (50 μM). Values are the mean ± SEM of 4 independent experiments.

CELLS

No treatment

A23187 (200 nM)

SKF 96365 (10 μM)

A23187 + SKF 96365

Ac-DEVD-CHO + A23187


Control (48 h)

398 ± 25

545 ± 51**

409 ± 17

524 ± 25*

522 ± 23*

Scott (48 h)

371 ± 5

382 ± 1

390 ± 7

371 ± 14

380 ± 10

Control (72 h)

361 ± 22

470 ± 25*

417 ± 31

490 ± 44*

520 ± 90*

Scott (72 h)

403 ± 62

408 ± 70

376 ± 46

354 ± 54

356 ± 43


*P < 0.05, **P < 0.01 versus the corresponding values obtained with non-treated cells.

Martínez and Freyssinet BMC Cell Biology 2001 2:20   doi:10.1186/1471-2121-2-20

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