[Ca2+]i determination in control and Scott cells by flow cytometry. Cells treated for 72 h by 200 nM A23187 or 10 μM SKF 96365 were loaded with the fluorochrome Fluo-3 (3 μM) and propidium iodide (PI, 10 μg/ml). Histograms show the number of cells as a function of Fluo-3 fluorescence for the PI negative cell population only. One representative experiment out of four performed likewise is shown.
Martínez and Freyssinet BMC Cell Biology 2001 2:20 doi:10.1186/1471-2121-2-20