Additional Fileset 8.

Fluorescent cargo clears the ER and accumulates in the Golgi prior to a high flux of Golgi exit These movies show the Golgi region of a PtK₂ cell expressing T2-CFP and VSVG3-YFP beginning 15 minutes at 32°C, prior to the period when the bulk of cargo exits the Golgi. The closeup movies (color | grey) show a twofold enlarged region. Most VSVG3-YFP has cleared the ER but some pre-Golgi TCs are still entering the Golgi. At this time, cargo completely fills Golgi elements, and cargo-specific regions are infrequent and small (closeup). In the color movie, VSVG3-YFP is in green, and T2-CFP in red, with overlay in yellow. Frame rate: 6 s/frame. Bar (greyscale): 5 μm.

Format: MOV Size: 7.2MBFormat: MOV Size: 7.2MBFormat: MOV Size: 7.2MBFormat: MOV Size: 7.2MB Download file

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White et al. BMC Cell Biology 2001 2:19   doi:10.1186/1471-2121-2-19