Additional Fileset 2.

Transfer of cargo during Golgi exit. VSVG3-YFP was pulsed into the secretory pathway as in Fig. 1B and imaging initiated after 27 minutes at 32°C. Fluorescence of cargo in Golgi elements becomes progressively fainter from 27 to 42 minutes of transport, but fluorescence of the Golgi resident T2-CFP remains the same. Quantitation of normalized, background subtracted fluorescence signals over time shows that the total level of VSVG3-YFP in the cell remains constant, but the level in the Golgi (region indicated by the dotted line in the top left panel) drops to below 60% of the initial level (Figure 2). VSVG3-YFP in Golgi elements is concentrated compared to VSVG3-YFP in the plasma membrane and so appears brighter in the Golgi. The movies have been split to comply with file size requirements. The order is indicated by the letter (A, B...). Full-length movies can be re-created in QuickTime Pro by copying the movies sequentially into a new movie window. In the color movies, VSVG3-YFP is in green, and T2-CFP in red, with overlay in yellow. Elapsed time is indicated as Hours:Minutes:Seconds (.fractions). Frame rate: 9.0 s/frame. Bar: 10 μm.

Format: MOV Size: 3MBFormat: MOV Size: 3MBFormat: MOV Size: 3MBFormat: MOV Size: 3MBFormat: MOV Size: 3MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

White et al. BMC Cell Biology 2001 2:19 doi:10.1186/1471-2121-2-19