Additional Fileset 1.

Golgi dynamics during a high flux of cargo exit. VSVG3-YFP was accumulated in the ER of transiently transfected PtK₂ cells by incubation at 40°C and transport initiated by shift to 32°C. Imaging was initiated after 29 minutes at 32°C, during the peak period of TC exit from the Golgi. Golgi elements containing T2-CFP remain stable as VSVG3-YFP cargo exits the Golgi in TCs, which track outwards to the cell periphery. In the color movie, VSVG3-YFP is in green, and T2-CFP in red, with overlay in yellow. Elapsed time is indicated as Hours:Minutes:Seconds (.fractions). Frame rate: 7.10 s/frame. Bar: 10 μm.

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White et al. BMC Cell Biology 2001 2:19   doi:10.1186/1471-2121-2-19