Figure 4.

Subcellular localization of Cx43. MC3T3-E1 cells were treated with A. PBS vehicle (control), B. BMP-2, or C. TGF-β1 for 48 h. Immunofluorescent localization of Cx43 visualized with epifluorescence microscopy (top panel). Immunoprecipitation of Cx43 in cytosolic (left lanes) and membranous (right lanes) fractions.