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Open Access Research article

Evidence for the involvement of Gi2 in activation of extracellular signal-regulated kinases in hepatocytes

Øyvind Melien1, Thoralf Christoffersen1 and Mouldy Sioud2*

Author Affiliations

1 Department of Pharmacology, Faculty of Medicine, University of Oslo, Box 1057 Blindern, N-0316, Oslo, Norway

2 Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway

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BMC Cell Biology 2001, 2:13  doi:10.1186/1471-2121-2-13

Published: 24 July 2001

Abstract

Background

Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins.

Results

Targeting the Gi expression by a specific ribozyme inhibited the PGF -induced ERK1/2 activation in hepatocytes. On the other hand a non-cleaving form of the Gi ribozyme did not significantly decrease the ERK1/2 activation. In ribozyme-treated cells the Gi protein level was reduced, while the G level was not affected thus confirming the specificity of the ribozyme.

Conclusion

The present data suggest an important role of Gi2 in PGF -induced ERK1/2 signaling in hepatocytes.