Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions
Laboratory of Environmental Biochemistry, Department of Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan
Division of Hematology, Longwood Medical Research Center 617, 221 Longwood Avenue, Boston, MA 02115, USA
BMC Cell Biology 2001, 2:10 doi:10.1186/1471-2121-2-10Published: 13 June 2001
Table 1: Methods of fixation and extraction for immunofluorescent microscopy.
Table 2: Comparison of the effects of different methods of preparation on the fluorescence intensity (Flu. int.), pattern of fluorescent labeling of stress fiber (SF) and morphological preservation of microextensions (ME) by TRITC-phalloidin (phalloidin) and monoclonal anti-β-actin antibody (mAb-β-actin) and pattern of tubulin staining by β-tubulin antibody (mAb-β-tubulin). Morphological preservation of the cells attached on coverslips (Morph.) and of microextensions (ME) were also examined by SEM before and after treatment for fluorescence microscopy. The range is from excellent (+++++) to very poor (0).
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