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Open Access Highly Accessed Research article

Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions

Fumihiko Nakamura

Author Affiliations

Laboratory of Environmental Biochemistry, Department of Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan

Division of Hematology, Longwood Medical Research Center 617, 221 Longwood Avenue, Boston, MA 02115, USA

BMC Cell Biology 2001, 2:10  doi:10.1186/1471-2121-2-10

Published: 13 June 2001

Additional files

Tables:

Table 1: Methods of fixation and extraction for immunofluorescent microscopy.

Table 2: Comparison of the effects of different methods of preparation on the fluorescence intensity (Flu. int.), pattern of fluorescent labeling of stress fiber (SF) and morphological preservation of microextensions (ME) by TRITC-phalloidin (phalloidin) and monoclonal anti-β-actin antibody (mAb-β-actin) and pattern of tubulin staining by β-tubulin antibody (mAb-β-tubulin). Morphological preservation of the cells attached on coverslips (Morph.) and of microextensions (ME) were also examined by SEM before and after treatment for fluorescence microscopy. The range is from excellent (+++++) to very poor (0).

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