Open Access Research article

Rapamycin promoted thrombosis and platelet adhesion to endothelial cells by inducing membrane remodeling

Ping Jiang1*, Yong Lan2, Jun Luo1, Ya-Li Ren3, Dong-Ge Liu4, Jian-Xin Pang4, Jin Liu5, Jian Li1, Chen Wang6 and Jian-Ping Cai1*

Author Affiliations

1 The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, No.1, DaHua Road, Dong Dan, Beijing 100730, P.R.China

2 Department of Vascular Surgery, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China

3 Laboratory of Electron Microscopy, Peking University First Hospital, Beijing 100034, China

4 Department of Pathology, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China

5 College of Lifesciences, Beijing Normal University, Beijing 100875, China

6 Department of Respiratory Medicine, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China

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BMC Cell Biology 2014, 15:7  doi:10.1186/1471-2121-15-7

Published: 24 February 2014

Additional files

Additional file 1: Figure S1:

(A) The time-course of effects of rapamycin on thrombotic occlusion in rats. Data exhibited that rapamycin promoted thrombotic occlusion with time. n = 5 in each group. (B) HUVECs were cultured on the gelatin-coated coverslips. Freshly isolated platelets, together with DMSO, were added to the coverslip and incubated for 30 minutes. Cells were fixed and stained with crystal violet. The coverslips were mounted and imaged by microscopy. The time course of platelet-endothelial adhesion was evaluated. Columns, means of three independent experiments; bars, SD. (C). Platelets attached on the endothelium at the site of ruffles (upper panel) and filopodialike protrusions (lower panel). HUVECs were cultured on the coverslips and then PKH26 labeled platelets were added into the medium. Cells were washed, fixed and stained with (upper panel) or without (lower panel) anti-β-actin antibody and the second fluorescent antibody. Then cells were analyzed with microscopy (lower panel) or immunofluorescence microscopy (upper panel).

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