Stem/progenitor cells analysis. A: Flow cytometry analysis of stem/progenitor cells isolated from foetal or adult livers. Cells were isolated by magnetic immunoselection for EpCAM. Flow cytometry analysis, where cells were stained with anti-EpCAM and anti-NCAM antibodies, demonstrated the enrichment of EpCAM + cells. A significant number of EpCAM + cells were also positive for NCAM. B: RNA analysis for SerpinB3 in human liver stem/progenitor cells. In the real time amplification for human SerpinB3 data were normalized to GAPDH housekeeping gene and the y-axis represents the relative mean mRNA level of the SB3 gene expressed by 2-ΔCt*10-5, with the bars representing the standard error. In the lower panel agarose gel electrophoresis of individual corresponding samples is shown as example.
Villano et al. BMC Cell Biology 2014 15:5 doi:10.1186/1471-2121-15-5