Additional file 3: Figure S3.
Nuclear and cytosolic fractionation of cell lysates from control ER-MXD3.E66D and experimental ER-MXD3 lines at different time points of tamoxifen treatment. Immunoblotting for HA shows that the fusion proteins disappear from the cytosolic fraction and subsequently become enriched in the nuclear fraction upon tamoxifen treatment. GAPDH was used as a loading control and marker of the cytosolic fraction; histone H3 was used as a loading control and marker of the nuclear fraction.
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Ngo et al. BMC Cell Biology 2014 15:30 doi:10.1186/1471-2121-15-30