MXD3 activation induces a transient increase in BrdU incorporation. Bromodeoxyuridine proliferation assay of ER-MXD3.E66D and ER-MXD3 cell lines treated with 1 μM 4-OHT normalized to vehicle. Treatments were staggered such that all conditions were assayed at the same time for BrdU incorporation after a 2 hour BrdU pulse. ER-MXD3 significantly (p = 0.0487, two-way ANOVA, n = 8) responds to 4-OHT treatment compared to vehicle, whereas ER-MXD3.E66D does not. Results of bonferroni post-test analysis comparing 4-OHT to vehicle are noted with asterisks.
Ngo et al. BMC Cell Biology 2014 15:30 doi:10.1186/1471-2121-15-30