Additional file 2.

T. cruzi epimastigotes incubated for 30 min at 16°C with Alexa Fluor 488-conjugated albumin and subsequently with anti TcCruzipain monoclonal antibody (detected with Alexa Fluor 594-conjugated secondary antibody). Albumin accumulates at the cell anterior, perpendicular to the kinetoplast (k), in a location that corresponds to the flagellar pocket region. Furthermore, albumin also localizes to the cell posterior, behind the nucleus (n). However, at 16°C albumin does not colocalize with TcCruzipain (a reservosomal marker). Therefore, this posterior labeling represents endocytic vesicles en route to reservosomes. Scale bar = 5 μm.

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Kalb et al. BMC Cell Biology 2014 15:23   doi:10.1186/1471-2121-15-23