Open Access Highly Accessed Research article

Phenotypic plasticity in normal breast derived epithelial cells

Candice AM Sauder1, Jillian E Koziel2, MiRan Choi3, Melanie J Fox2, Brenda R Grimes2, Sunil Badve4, Rachel J Blosser1, Milan Radovich12, Christina C Lam5, Melville B Vaughan5, Brittney-Shea Herbert2 and Susan E Clare3*

Author Affiliations

1 Department of Surgery, Indiana University School of Medicine, 980 W. Walnut Street, Indianapolis, IN 46202, USA

2 Department of Medical and Molecular Genetics, Indiana University School of Medicine, 975 W. Walnut Street, Indianapolis, IN 46202, USA

3 Department of Surgery, Feinberg School of Medicine, Northwestern University, 303 E. Superior Street, Chicago, IL 60611, USA

4 Department of Pathology, Indiana University School of Medicine, 350 West 11th Street, Indianapolis, IN 46202, USA

5 Department of Biology, University of Central Oklahoma, 100 North University Drive, Edmond, OK 73034, USA

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BMC Cell Biology 2014, 15:20  doi:10.1186/1471-2121-15-20

Published: 10 June 2014

Additional files

Additional file 1: Table S1:

Age, ethnicity and Gail breast cancer risk estimates of tissue donors. Table S2. Immunohistochemistry protocols.

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Additional file 2:

Methods.

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Additional file 3: Figure S1:

Immunohistochemistry of the cells in Matrigel® sandwiches showing squamous differentiation. Figure S2. Hematoxylin and eosin stained sections of breast tissue adjacent to the core utilized for the production of epithelial and stromal cells. Figure S3. Phase contrast photomicrograph of cell colony grown from a single cell (Evos x1, Advanced Microscopy Group, Bothell, WA; 40x objective). Figure S4. A. Tartrate-resistant acid phosphatase staining of K-HME 511 cells grown on laminin (40x). Positive staining cells are in the minority. B. FACS analysis of K-HME 511 cells. PE = anti CD151; ACP = anti-Calcitonin R(recptor). Figure S5. Telomerase activity of K-HME and K-HMS cells grown in different media (WIT-P, DMEM/F12, and MEGM) using the polymerase chain reaction (PCR)-based TRAP-eze assay. Figure S6. Graphical representation of quantitative PCR results.

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