Phenylalanine incorporation into proteins in myotubes depleted of PDCD4. (A) PDCD4 abundance in cells treated with scrambled siRNA or siRNA oligonucleotides designed against PDCD4. (B) Cells treated with scrambled (SC) or PDCD4 siRNA #2 were cultured in differentiation (Ctl) or serum-free medium (Stv) for 12 h. Phenylalanine incorporation into proteins was then measured. Within each nutrition group, cells depleted of PDCD4 were compared to those treated with scrambled siRNA using paired t-test. Means ± SE of 5–6 independent experiments.
Kakade et al. BMC Cell Biology 2014 15:2 doi:10.1186/1471-2121-15-2