Interaction of PDCD4 with eIF4A or eIF4G in myotubes. Myotubes were incubated in differentiation (Ctl) or starvation (serum- and amino acid-free) medium for 12 h. They were then harvested (Stv) or refed in the differentiation medium in the presence of rapamycin for 1 or 3 h. Lysates were immunoprecipitated with either (A) anti eIF4A or (C) anti PDCD4 antibodies followed by western blotting. Signals from immunoprecipitates were quantified and shown in B, D and E. Means ± SE; n = 4; bars with different letters differ (P < 0.05).
Kakade et al. BMC Cell Biology 2014 15:2 doi:10.1186/1471-2121-15-2