Figure 1.

In L6 myotubes, PDCD4 abundance, but not phosphorylation, is sensitive to medium nutrient composition and requires mTORC1 function. L6 Myotubes were incubated in differentiation (Ctl) or starvation medium (serum-free, amino acid-free RPMI medium) for 12 h. They were then harvested (Stv) or refed in the differentiation medium supplemented with DMSO (−) or 50 nM rapamycin (+) for 1 or 3 h. (A) Phosphorylated (ph) and total PDCD4 (Ser67), ph-S6K1 (Thr389) and γ-tubulin were analyzed by immunoblotting. (B and C) phosphorylated and total PDCD4 signals were expressed relative to γ tubulin. Means ± SE of 5–6 independent experiments; bars with different letters differ (P < 0.05).

Kakade et al. BMC Cell Biology 2014 15:2   doi:10.1186/1471-2121-15-2
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