Figure 5.

Characterization of Embryoid Bodies (EBs) for PcG gene transcripts. (A) Expression of PRC1 (RING1A, RING1B, BMI1and CBX2) in Day 7 and Day 15 EBs grown in suspension culture. (B) Expression of PRC2 (EZH2, SUZ12, EED and JARID 2) gene transcripts in Day 7 and Day 15 EBs grown in suspension culture. (C) Expression of PRC1 (RING1A, RING1B, BMI1and CBX2) gene transcripts at Day 10 in EBs cultured on gelatin coated dishes. (D) Expression of PRC2 (EZH2, SUZ12, EED and JARID 2) gene transcripts at Day 10 in EBs cultured on gelatin coated dishes. The expression is relative to undifferentiated KIND1 cells (set as 1). Error bars represent ± SEM, statistical significance represented as *(p < 0.05), **(p < 0.02) and ***(p < 0.001).

Pethe et al. BMC Cell Biology 2014 15:18   doi:10.1186/1471-2121-15-18
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