Additional file 2: Figure S1.
Characterization of fetal Mesenchymal Stem/Stromal Cells. A) Light microscopy (x10 magnification) of fetal MSC showing an adherent, fibroblast like morphology. Fetal MSC display osteogenic (B), adipogenic (C) and chondrogenic (D) differentiation capacity after incubation with specific induction media and staining with Alizarin Red, Oil red-O or Alcian Blue respectively (x4, x10 and x2 magnification respectively). E) Flow cytometry for MSC positive markers: CD73, CD105, CD90, CD44, HLA-ABC, CD29, CD49b, CD49d. F) Flow cytometry for MSC negative markers: CD11b, CD34, CD45, CD117, CD31, HLA-DR, CD14. Fluorophore conjugates are indicated.
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Pelekanos et al. BMC Cell Biology 2014 15:15 doi:10.1186/1471-2121-15-15