Treatment with NaHS modulates protein synthesis and degradation pathways in cultured brain slices from ZDF (zucker diabetic fatty rat). (A) The expression of mTOR and its phosphorylated form (p-mTOR2448) is higher in ZDF and is lowered to normal levels by H2S treatment. (B) The expression of S6 protein and its phosphorylated form (p-S6 ribosomal protein) is higher in ZDF rats and is normalized by NaHS treatment. (C) Increased mTOR activity in ZDF is counteracted by NaHS. (D) Increased S6 protein activity in ZDF is counteracted by NaHS. (E) LC3-I is higher in ZDF brains while LC3-II shows lower expression. NaHS lowers LC3-I expression. (F) Autophagy levels are lower in cultured ZDF slices compared to Lean as shown by LC3-II/LC3-I ratio. NaHS increases autophagy levels in ZDF. (G) Mono- and polyubiquitinated protein expression is 3 times higher in ZDF brains compared to Lean and is lowered to normal by NaHS. Western blot data showing protein of interest (upper part) and β-actin (lower part) from the same samples. Data are means ± SEM (n ≥ 5 per group), * < 0.05 = different from control gray bars in each group; One way ANOVA. Western blot expression is normalized to β-actin; lanes of western blot insets are in the same order as in the X-axis.
Talaei et al. BMC Cell Biology 2014 15:1 doi:10.1186/1471-2121-15-1