Observation and analysis of CD44+CD117+CSCs. The CD44+CD117+cells were sorted from the SKOV-3 cell line by MACS, and cultured in the stem cell culture medium on day 20 passage (A1, 100×, A2, 400×) or common 2D culture on day 10 passages (A3, 100×). B1 and B2 show the CD44 and CD117 positive cells detected by FCM. B3 displays the image of CD44+CD117+CSCs without staining with immunofluorescence (400×). Consistent with the result of immunofluorescence analysis, FCM analysis shows a high percentage of CD44 and CD117 molecules (95.04%) in the CD44+CD117+cells after being in the stem cell culture medium for 2 day (C Left); the percentage was only 4.16 for the SKOV-3 cells (C Right).
Chen et al. BMC Cell Biology 2013 14:7 doi:10.1186/1471-2121-14-7