GxcC binds to a subset of Dictyostelium Rac proteins. (A) GST and various purified GST-Rac proteins were incubated with GFP-GxcC cell lysate. Beads were precipitated and the amount of GFP-GxcC and bait was detected by western blotting using antibody specific for GFP or GST, respectively (B) The amount of detected proteins was quantified using ImageJ and normalized to the amount of GxcC protein used in the assay. Shown is the percentage of bound GxcC as mean and SEM of three independent experiments.
Plak et al. BMC Cell Biology 2013 14:6 doi:10.1186/1471-2121-14-6