Effect of Spca2 silencing on basal Ca2+ in HC11 cells. Calcium assays were conducted to assess the basal Ca2+ influx in proliferating and differentiated HC11 cells treated with siNT or siSpca2. A Basal Ca2+ influx is shown as a trace of mean fluorescence relative to baseline in proliferating and differentiated HC11 cells treated with siNT and siSPCA2. B Rate of basal Ca2+ influx, calculated as the slope between 0–5 s after 2 mM Ca2+ addition, in differentiated and proliferating HC11 cells with siNT or siSpca2. Rate is expressed as a percentage relative to differentiated cells treated with siNT and CPA (n = 4; mean ± SD, *P < 0.05).
Ross et al. BMC Cell Biology 2013 14:57 doi:10.1186/1471-2121-14-57