Table 3

Primer sequences
Name Sequence (forward) Sequence (reverse) Product size (bp)
pGAPDH 5′-ggg cat gaa cca tga gaa gt-3′ 5′-agg cag gga tga tgt tct gg-3′ 230
pOat1 5′-tac act ggg gag ctg tac cc-3′ 5′-tct ttt cct cct gct ttc ca-3′ 246
pOat3 5′-ctt cct gtt gtc ctg gtg gt-3′ 5′-ggg aaa gac aga ggg tca ca-3′ 248
pMdr1 5′-tcc agt ttc ctt ttg gag ga-3′ 5′-tgt cct gtc gtt tgg ttt ca-3′ 176
pMrp1 5′-gca gat cac cgc ata ctt ga-3′ 5′-gtc cag gtc gtc tcg gta ac-3′ 219
pMrp2 5′-gct tgc agt tcg tct gga gt-3′ 5′-caa cag cca caa tgt tgg tc-3′ 191
pOatp1a2 5′-atg ggc cta ggg tgt ttc tt-3′ 5′-agg cat gat ggg agt ttc ac-3′ 246

Primer design was performed on the basis of sequence data available at NCBI (http://www.ncbi.nlm.nih.gov webcite) using Primer3 V0.2 software [47], which is online available (http://www.primer3.sourceforge.net webcite) with default settings.

Heussner and Dietrich

Heussner and Dietrich BMC Cell Biology 2013 14:55   doi:10.1186/1471-2121-14-55

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