Identification of markers differentially expressed between cultures rich in mesenchymal stem cells and non-stem cell mesenchymal cell cultures. A: Analysis of antibody array and proteomics data identified markers upregulated in cultures rich in mesenchymal stem cells (bone marrow – BM; and breast adipose - BA), compared with non-stem cell mesenchymal cell cultures (olfactory tissue – OT). Antibody array and proteomics data also identified markers upregulated in non-stem cell mesenchymal cultures (OT) compared with cultures rich in mesenchymal stem cell (BM and BA). B: qPCR was carried out on all tissues (N = 3/cell type) for markers identified in A. Only markers found to have expression profiles consistent with A are represented (NFL: neurofilament light polypeptide; END: endoplasmin). ΔΔCt values were calculated relative to GAPDH and were normalized relative to the tissue with the highest expression. Standard error is displayed - only significant differences between tissue types, consistent with A, (P < 0.05) are indicated (*).
Wetzig et al. BMC Cell Biology 2013 14:54 doi:10.1186/1471-2121-14-54