Phenotypic analysis of tissue cells by flow-cytometry and immuno-fluorescence. A: Bone marrow (BM), breast adipose (BA), olfactory tissue (OT) and foreskin fibroblast (FF) cells were analyzed by flow-cytometry for the expression of 17 extracellular markers and the percentage of positive cells calculated compared with unstained controls (N = 3/cell type). Standard error is presented and significant differences between tissue types (P < 0.05) are indicated (*). B: Tissue cells were analyzed for the expression of fibroblast-associated markers; fibroblast surface protein (FSP) (C -olfactory tissue cells), Collagen 3 (Coll 3) (D – bone marrow cells ) prolyl-4-hydroxylase, beta polypeptide (P4HB) (E – breast adipose cells), and Collagen 1 (Coll 1) (F – foreskin fibroblast cells) using immuno-fluorescence. Scale bar (F) = 50 μm - applicable to C, D and E. The percentage of positive cells was calculated compared with no primary antibody controls (N = 3/cell type). Standard error is demonstrated - no significant differences between tissue types were found.
Wetzig et al. BMC Cell Biology 2013 14:54 doi:10.1186/1471-2121-14-54