Impact of the knockdown of the α6 integrin subunit on the survival of human IECs. A. Representative (n ≥ 3) WB analyses of α6 and actin from adhering HIEC cell cultures, following their infection by a lentivirus carrying GFP (control), shCNS or shα6. Specific antibodies for α6 and actin were used. B. Adhering HIEC cell cultures were infected as in (A), except that ISEL assays were performed and compared to controls. C. Adhering HIEC cell cultures were infected as in (A), except that CASP-3 relative activity was established, by comparison to controls. A-C. Results obtained with -2PC Caco-2/15 cells were highly similar to those shown here for HIEC cells. B-C. Statistically significant (0.0001 ≤ P ≤ 0.001; n ≥ 3) differences between treated and control cultures are indicated by (*). Statistically significant (0.0005 ≤ P ≤ 0.005; n ≥ 3) differences between shα6 and shCNS are indicated by (#).
Beauséjour et al. BMC Cell Biology 2013 14:53 doi:10.1186/1471-2121-14-53