Contributions of the α2, α3, and α5 integrin subunits in the engagement of Fak and Src, in human IECs. A. Representative (n ≥ 3) WB analyses of Src and Fak, and verifications of Fak-Src interactions, from adhering HIEC (Undifferentiated) and 30PC Caco-2/15 (Differentiated) cell cultures maintained 24 h serum-free (control) with mouse IgG’s, P1E6 (α2 binding activity-blocking mAb), P1B5 (α3 binding activity-blocking mAb), or P1D6 (α5 binding activity-blocking mAb). Specific antibodies for pY576/577p125Fak, pY418p60Src and pY397p125Fak, as well as for respective total protein forms, were used. B-C. HIEC (Undifferentiated; filled columns) and 30PC Caco-2/15 (Differentiated; open columns) cell cultures were maintained and processed as in (A), except that the relative pY576/577 levels of Fak (B), as well as the relative activation levels of Src (C) and Fak (D), were established in comparison to controls. A-D. Results obtained with -2PC Caco-2/15 cells were highly similar to those shown here for HIEC cells. B-D. Statistically significant (0.0001 ≤ P ≤ 0.001; n ≥ 3) differences between treated and control cultures are indicated by (*). Statistically significant (0.0005 ≤ P ≤ 0.005; n ≥ 3) differences between differentiated and undifferentiated IECs are indicated by (#).
Beauséjour et al. BMC Cell Biology 2013 14:53 doi:10.1186/1471-2121-14-53