Engagement of Fak and Src by β1 and β4 integrins in the suppression of human IEC anoikis. A. Representative (n ≥ 4) WB analyses of the activation of Src and Fak, and verifications of Fak-Src interactions, from -2PC (Undifferentiated) and 30PC (Differentiated) Caco-2/15 adhering cell cultures maintained 24 h serum-free (control) with mouse IgG’s, P4C10 (β1 binding activity-blocking mAb), or 3E1 (β4 binding activity-blocking mAb). Specific antibodies for pY576/577p125Fak, pY418p60Src and pY397p125Fak, as well as for respective total protein forms, were used. B-D. -2PC (Undifferentiated; filled columns) and 30PC (Differentiated; open columns) Caco-2/15 cell cultures were maintained and processed as in (A), except that the relative pY576/577 levels of Fak (B), as well as the relative activation levels of Src (C) and Fak (D), were established in comparison to controls. A-D. Results obtained with HIEC cells were highly similar to those shown here for -2PC Caco-2/15 cells. B-D. Statistically significant (0.0001 ≤ P ≤ 0.001; n ≥ 4) differences between treated and control cultures are indicated by (*). Statistically significant (0.0005 ≤ P ≤ 0.005; n ≥ 4) differences between differentiated and undifferentiated IECs are indicated by (#).
Beauséjour et al. BMC Cell Biology 2013 14:53 doi:10.1186/1471-2121-14-53