Human IECs exhibit differentiation state-associated distinctions in anoikis sensitivity. A. Representative (n ≥ 3) time-course kinetics of CAD-mediated DNA laddering from -2PC (Undifferentiated; lanes 1-5) and 30PC (Differentiated; lanes 6-10) Caco-2/15 cells maintained 0-24 h in suspension, serum-free. L, 100-bp DNA size markers. B. Time-course kinetics (n ≥ 3) of caspase activity for CASP-3 (filled diamonds), CASP-8 (open circles) and CASP-9 (open squares), from -2PC Caco-2/15 cells maintained as in (A). C. Same as in (B), except that CASP-8, -9 and -3 activities were assessed from 30PC Caco-2/15 cells maintained as in (A). A-B. Results obtained with HIEC cells were highly similar to those shown here for -2PC Caco-2/15 cells.
Beauséjour et al. BMC Cell Biology 2013 14:53 doi:10.1186/1471-2121-14-53