Open Access Highly Accessed Research article

BORIS/CTCFL is an RNA-binding protein that associates with polysomes

Babatunji W Ogunkolade1, Tania A Jones1, Johan Aarum1, Jaroslaw Szary1, Nicholas Owen1, Diego Ottaviani1, Muhammad A Mumin1, Shyam Patel1, Christopher A Pieri1, Andrew R Silver2 and Denise Sheer1*

Author Affiliations

1 Centre for Neuroscience and Trauma, Queen Mary University of London, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, E1 2AT, UK

2 Centre for Digestive Diseases, Queen Mary University of London, Blizard Institute, Barts and The London School of Medicine and Dentistry, 4 Newark Street, London, UK

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BMC Cell Biology 2013, 14:52  doi:10.1186/1471-2121-14-52

Published: 26 November 2013

Additional files

Additional file 1: Figure S1:

Endogenous BORIS co-localises with newly synthesized RNA. HEK293T cells were pulse-labelled with 5’Fuorouridine (5’FU) for 10 minutes to label nascent RNA. Double immunoflorescence was performed with an anti-BrdU antibody to detect 5’FU (Red) and ant-BORIS (Green) as indicated. Nuclei were visualised with DAPI (blue).

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Additional file 2: Figure S2:

Partial PCR amplification of endogenous BORIS in hNP1 cells and HEK293T cells. Gel electrophoresis analysis of fragments used for sequencing amplified with (A) primers +67F and +633R as previously described [22] or (B) with primers BORIS exon 9-10-11 forward: 5’-TGACCGCTCACATTCGTACC-3’ and reverse 5’-AGTGAACACGCAACCCGAAT-3’.

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Additional file 3: Table S1:

Probes and transcripts associated with BORIS in hNP1 cells.

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Additional file 4: Table S2:

Probes and transcripts associated with BORIS in hNP1 cells differentiated to neurons over 6 days (6dN).

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Additional file 5: Table S3:

BORIS-associated transcripts up- or down-regulated during neural differentiation.

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Additional file 6: Figure S4:

BORIS associates with RNA transcripts in stem cells and young neurons. BORIS associates with several transcripts (coloured blue) of the WNT signalling pathway.

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Additional file 7: Figure S5:

Effects on protein levels after BORIS overexpression in HEK293T cells. Images and the associated densitometry measurements used to assess the protein levels of WNT5A/B and TCF3 after BORIS overexpression.

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Additional file 8: Figure S3:

Confirmation of BORIS antibody specificity. Immuno-blotting of oligo-dT-RNA bound protein complexes from HEK293T cells transiently expressing CTCF (C3-CTCF), BORIS (C3-BORIS) or empty vector (C3-empty). Blot probed with anti BORIS antibodies.

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