Immunolocalization of MAST on testis and sperms of normal males. (A) Immunolocalization of MAST protein onto testicular sections using indirect immunofluorescence. Localization of MAST antibody was detected by rabbit polyclonal secondary antibody conjugated with Alexa fluor 488. Propidium Iodide (PI) was used as the counterstain. Merging of the Alexa and PI captures on a confocal microscope shows cytoplasmic localization of the protein in all the cell types in testis (XY). Intense staining is observed in elongated spermatids present towards the lumen of the seminiferous tubules. Pre-immune serum was used as the negative control; magnification was 100×; scale bar = 25 μm. (B) Immunolocalization on sperm: Immunolocalization with antibody to MAST protein showed that in sperms the protein localized to the sperm head with acrosomes showing intense staining. Protein was also present on sperm tails. DAPI is used as the counterstain for nucleus and pseudo color red is used for representation. Magnification- 63×; scale bar = 10 μm.
Bhattacharya et al. BMC Cell Biology 2013 14:50 doi:10.1186/1471-2121-14-50