Open Access Research article

Collagen/β1 integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cells

Di Zeng, Dong-Bo Ou, Ting Wei, Lu Ding, Xiong-Tao Liu, Xin-Lin Hu, Xue Li and Qiang-Sun Zheng*

Author Affiliations

Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, China

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BMC Cell Biology 2013, 14:5  doi:10.1186/1471-2121-14-5

Published: 25 January 2013

Additional files

Additional file 1: Figure S1:

Expression of markers related to three germ layer within EBs. Semiquantitative RT-PCR measurement of markers related to the three germ layer (endoderm: a-Fetoprotein, GATA-6; mesoderm: Brachyury; ectoderm: TuJ1, Map2) within 3d- and 5d-EBs derived from cells subject to integrin disruption and controls. Experiments were performed in triplicate, and the transcripts for GAPDH were used for internal normalization.

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Additional file 2: Figure S2:

Loss of pluripotency within EBs after integrin disruption. Semiquantitative RT-PCR (A) and quantitative PCR (B, C) measurement of pluripotent markers (OCT3/4 and Nanog) within 3d- and 5d-EBs derived from cells subject to integrin disruption and controls. Expression levels of each gene were normalized to GAPDH. Mean fold change relative to GAPDH and SD from triplicate experiments are shown.

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Additional file 3: Table S1:

Primers and cycling conditions for RT-PCR.

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Additional file 4: Table S2:

Primers for RT-PCR in supplementary figures.

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