Table 1

MS analysis of 20 (19) differential protein spot species in pHPL and tPRPa) b)
Ratio ‘pHPL’/‘tPRP’ Spot Protein name SwissProt ID Sequence coverage (%) Peptides queries matched Percental match (%) MOWSE score (> 56; p < 0.05) MW (Da) pI
0.62 1 Apolipoprotein A-I sp|P02647|APOA1_HUMAN 80 29 33.7 292 30759 5.56
0.65 2 Apolipoprotein A-I sp|P02647|APOA1_HUMAN 64 23 26.4 205 30759 5.56
0.69 3 Retinol-binding protein 4 sp|P02753|RET4_HUMAN 67 11 15.3 114 23337 5.76
0.71 4 Angiotensinogen sp|P01019|ANGT_HUMAN 19 9 14.8 62 53406 5.87
0.71 5 CD5 antigen-like sp|O43866|CD5L_HUMAN 28 8 9 66 39603 5,28
0.74 6 Apolipoprotein A-I sp|P02647|APOA1_HUMAN 59 23 29.5 193 30759 5.56
1.55 7 Vitamin D-binding protein sp|P02774|VTDB_HUMAN 37 15 35.7 147 54526 5.40
1.84 8 Hemopexin sp|P02790|HEMO_HUMAN 25 9 10.1 68 52385 6.55
2.68 9 Fibrinogen gamma chain sp|P02679|FIBG_HUMAN 59 21 25.3 201 52106 5.37
2.77 10 Fibrinogen gamma chain sp|P02679|FIBG_HUMAN 56 21 23.1 226 52106 5.37
2.98 11 Tropomyosin alpha-4 chain sp|P67936|TPM4_HUMAN 33 10 17.2 83 28619 4.67
3.35 12 Fibrinogen gamma chain sp|P02679|FIBG_HUMAN 27 13 15.1 88 52106 5.37
4.30 13 Tubulin beta chain sp|P07437|TBB5_HUMAN 39 17 20.7 149 50095 4.78
4.59 14 Fibrinogen gamma chain sp|P02679|FIBG_HUMAN 56 21 24.1 235 52106 5.37
6.00 15 Fibrinogen gamma chain sp|P02679|FIBG_HUMAN 51 19 22.1 189 52106 5.37
6.69 16 Fibrinogen gamma chain sp|P02679|FIBG_HUMAN 27 11 16.7 73 52106 5.37
10.36 17 Chloride intracellular channel protein 1 sp|O00299|CLIC1_HUMAN 43 9 18 88 27248 5.09
10.76 18 Actin, cytoplasmic 1 sp|P60709|ACTB_HUMAN 53 16 22.2 134 42052 5.29
11.01 19b) Annexin A5 sp|P08758|ANXA5_HUMAN 21 5 10.4 53b) 35971 4.94
12.27 20 Actin, cytoplasmic 1 sp|P60709|ACTB_HUMAN 52 18 27.3 163 42052 5.29

a)Mascot software analysis by using SwissProt data base. Ratio pHPL/tPRP: <1 higher abundant in tPRP, >1 higher abundant in pHPL. PMF criteria: human source, one missed tryptic cleavage, methionine oxidation, score limit >56. b)Despite the fact that potential MS identification of Annexin A5 is below required scoring (5.6% less), this non-significant MS hit was included into the list because the suggested high ratio and protein ID was confirmed by Western blotting.

Kinzebach et al.

Kinzebach et al. BMC Cell Biology 2013 14:48   doi:10.1186/1471-2121-14-48

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