Table 3

List of Primers used in qRT-PCR
Gene 5′- 3′ nucleotide sequence
mBMP-2 F TCTTCCGGGAACAGATACAGG
mBMP-2 R TGGTGTCCAATAGTCTGGTCA
mOsterix F ATGGCGTCCTCTCTGCTTG
mOsterix R TGAAAGGTCAGCGTATGGCTT
mRunX-2 F GACTGTGGTTACCGTCATGGC
mRunX-2 R ACTTGGTTTTTCATAACAGCGGA
mDlx5 F CACCACCCGTCTCAGGAATC
mDlx5 R GCTTTGCCATAAGAAGCAGAGG
mTGFb1 F CTCCCGTGGCTTCTAGTGC
mTGFb1 R GCCTTAGTTTGGACAGGATCTG
mSMAD-2 F ATGTCGTCCATCTTGCCATTC
mSMAD-2 R AACCGTCCTGTTTTCTTTAGCTT
mTGFbR2 F CCGCTGCATATCGTCCTGTG
mTGFbR2 R AGTGGATGGATGGTCCTATTACA
mTwist1 F GGACAAGCTGAGCAAGATTCA
mTwist1 R CGGAGAAGGCGTAGCTGAG
mSox9 F GAGCCGGATCTGAAGAGGGA
mSox9 R GCTTGACGTGTGGCTTGTTC
mCol4a1 F CTGGCACAAAAGGGACGAG
mCol4a1 R ACGTGGCCGAGAATTTCACC
mCol-1 F GCTCCTCTTAGGGGCCACT
mCol-1 R ATTGGGGACCCTTAGGCCAT
mCol-2 F CAGGATGCCCGAAAATTAGGG
mCol-2 R ACCACGATCACCTCTGGGT

List of primers used to amplify the cDNA in order to investigate changes in mRNA upon BMP2 treatment in qRT-PCR. F: forward; R: reverse.

Halcsik et al.

Halcsik et al. BMC Cell Biology 2013 14:47   doi:10.1186/1471-2121-14-47

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