Cofilin suppression enhances MTLn3 cell adhesion. A. MTLn3 cells were stained for focal adhesion with mouse anti-paxillin primary antibody. The size and number of focal adhesions per unit area (40 μm2; red box) were measured in Metamorph, an average of 8 unit areas (boxes) at the leading edge of each cell were measured. B. Quantification of total area occupied by focal adhesion (μm2)/40 μm2 as: average number of focal adhesions/μm2 X average size of focal adhesions. n ≥ 25 cells in each experiment, three independent experiments. C. Cells were subjected to collagen I adhesion assay. Average values of cells adherent to plastic (not shown) were subtracted from average values adherent to collagen I. Average values of control cells were reported to 100%. Four independent experiments each performed in triplicate. ** p < 0.01 versus control. Scale bar: 10 μm.
Tahtamouni et al. BMC Cell Biology 2013 14:45 doi:10.1186/1471-2121-14-45