Analyses of spheres derived from HONE1 hybrid cells. A. RT-PCR analyses show that the expression of genes that encode CSC markers, CD9, CD24, CD44, CD90, and CD133, in sphere-forming cells (spheres A, B, and C) are progressively up-regulated compared to HONE1 and HONE1 hybrid cells. B. and C. Wnt Signaling Pathway qPCR Array assay lists the 34 up-regulated genes and 12 down-regulated genes in spheres, compared to HONE1 hybrid MCH4.4.
Cheng et al. BMC Cell Biology 2013 14:44 doi:10.1186/1471-2121-14-44