Substitution of Ser846 prevents effects of PP6c knockdown on E-cadherin localization. Either myc tagged mouse E-cadherin wild type (WT) or S846A were inserted into the vector with non-coding (Con.) or PP6c shRNA for stable expression in Caco-2 cells by lentiviral infection. Co-expression of myc-E-cadherin and shRNA were induced after cell confluence. (A) Immunofluorescent staining of myc-E-cadherin. (B) Quantification of fluorescence intensity of myc-E-Cadherin in (A) by line scans (10 μm). (C) The full width at half maximum (FWHM) of each line scan was calculated and the average of 38 scans  are presented (mean + SE).
Ohama et al. BMC Cell Biology 2013 14:42 doi:10.1186/1471-2121-14-42