Mitochondrial ROS generation following uncoupling by K+ ionophore or protonophore. (A) Representative traces of simultaneous measurements of mitochondrial H2O2 production rates, assessed by following the oxidation of Amplex Red to the fluorescent product resorufin, and respiration rates following sequential additions of vehicle (ethanol, control), valinomycin (Val) and CCCP. The resulting concentrations of Val and CCCP are depicted below the x-axis at the respective time intervals. (B) The quantitative effects of increasing K+ and H+ conductances on ROS generation are illustrated by plotting the rates of mitochondrial H2O2 production against the increased respiration rates for the respective concentrations of Val and CCCP. Values are means ± S.E.M. * and # indicate p < 0.05 compared to the initial value with no uncoupling agent present for H2O2 production rate and respiration respectively.
Morota et al. BMC Cell Biology 2013 14:40 doi:10.1186/1471-2121-14-40