Figure 2.

PKCθ regulates the myogenic program. A) Light microscopy images taken at 10X of scramble and PKCθshRNA cells from Day 0 through Day 4 of differentiation. On Day 4 cells were stained for MHC expression (green) and nuclei counterstained with DAPI (Blue). B) Time course of myogenin gene expression in differentiating scramble and PKCθshRNA cells. ***; P<0.0001 between scramble and PKCθshRNA cells at indicated time points. C) MHC protein expression in scramble and PKCθshRNA day 4 myotubes normalized to β-actin. ***; P<0.0002. D) Quantification of the density of MHC+ cells. **; P=0.0015. E) Number of nuclei per MHC+ cell. ***; P=0.0008. F) Time course of Focal adhesion kinase (FAK) gene expression in differentiating scramble and PKCθshRNA cells. **; P<0.01 and ***; P<0.001 between scramble and PKCθshRNA at indicated time points. G) Time course of caveolin 3 gene expression in differentiating scramble and PKCθshRNA cells. ***; P<0.001 at indicated time point. Data ± SEM. n=3 for all experiments.

Marino et al. BMC Cell Biology 2013 14:39   doi:10.1186/1471-2121-14-39
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