Additional file 9.

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Ric10 cells were cultured for 24 h in pmDM and then incubated for 20 min in pmDM supplemented with 100 μM vanadate. The cells were sequentially observed by time-lapse recording (Additional file 8). Then the medium was switched to pmDM supplemented with both 100 μM vanadate and 10 μM SU6656 and sequentially observed by time-lapse recording. Images were recorded every 2.5 min by phase-contrast time-lapse microscopy. Membrane ruffling in pmDM supplemented with vanadate (Additional file 8) was not suppressed in pmDM supplemented with SU6656 and vanadate.

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Mukai and Hashimoto BMC Cell Biology 2013 14:37   doi:10.1186/1471-2121-14-37