Additional file 4.
Effects of Src kinase inhibitor or protein tyrosine phosphatase inhibitor vanadate on fusion–related proteins of myogenic cells. Ric10 cells were cultured for 24 h in pmDM and then cultured in pmDM supplemented with 0.1% DMSO (−) or SU6656 (+) for 9 h (A), or vanadate (1, 10, 100 μM) for 24 h (B). Total lysates (20 μg of proteins) were subjected to immunoblot analyses. MyHC, myosin heavy chain; p120PY228, tyrosine phosphorylated p120; p120PT310, threonine phosphorylated p120. β-tubulin was used as a loading control.
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Mukai and Hashimoto BMC Cell Biology 2013 14:37 doi:10.1186/1471-2121-14-37