M-cadherin and p120 catenin form protein complex at lipid raft during myogenic differentiation. GGS25 cells were cultured in pmDM for 24 h at low cell density to avoid cell-cell contact. Cells were subjected to fractionation of the detergent-resistant membrane (DRM) fractions. (A) The distribution of M-cadherin in each fraction (30 μl) of growing (pmGM) or differentiating (pmDM) GGS25 cells was analyzed by immunoblotting with M-cadherin antibody. The line represents the detergent-resistant membrane fractions. (B) The distributions of M-cadherin, flotillin, and GM-1 in DRM fractions of differentiating cells are represented as the % of the amount in DRM fraction of growing cells. M-cadherin and flotillin were analyzed by immunoblotting using the appropriate antibodies. GM-1 was analyzed by dot blot assay with HRP-labeled CTB. Flotillin and GM-1 were used as markers of lipid rafts. Averages and standard deviations (n = 3) are shown and analyzed using Student’s t-test. *P < 0.1, **P < 0.05.
Mukai and Hashimoto BMC Cell Biology 2013 14:37 doi:10.1186/1471-2121-14-37