Additional file 1: Figure S1.
Cysteine cathepsin activity is similar in live cells and lysate. Human primary CD4 T cells were grown in R10-IL2. 5 × 104 cells resuspended in PBS was added to each well. For lysate, the volume of the lysate derived from 5 × 104 cells was calculated and added to each well. When using inhibitors, E64 was added to the well and preincubated for 30 min at 37C. Following incubation, the activity of an omnicathepsin substrate was measured. Average and SD of 3 experiments.
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Vaithilingam et al. BMC Cell Biology 2013 14:35 doi:10.1186/1471-2121-14-35