Additional file 1: Figure S1.
Cryopreservation does not significantly affect BMI-ASC osteogenic and adipogenic potential in vitro. BMI-ASCs that were freshly isolated or frozen and thawed, were induced into a. b. adipogenesis for 15 days or c. d. osteogenesis for 16 days, as described in the materials and methods section. Oil-red-o staining was used to evaluate adipogenic potential, and Alizarin red stain was used to determine ECM deposition. Quantification of e. ORO and f. ARS reflected no significant difference in adipogenic or osteogenic potential, respectively, in freshly isolated cells compared to frozen cells. Values reported as N ± SE.
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Frazier et al. BMC Cell Biology 2013 14:34 doi:10.1186/1471-2121-14-34