Expression of alkaline phosphatase mRNA indicated that higher BMI was associated with reduced ASC osteogenesis capacity in vitro. ASC osteogenesis was induced using a cocktail medium for 9 days. RT-PCR was performed using primers and a probe to alkaline phosphatase for a measure of mRNA expression. ΔCT values were normalized to internal GAPDH. a. ungrouped averages of triplicates b. grouped averages of ‘lean’ (BMI <25 n = 5) and ‘overweight’ (BMI >25; n = 5) BMI-ASCs. c. Linear regression analyses of differentiation data that was analyzed at day 9 using least squares ordinary fit. R2 value reflected a strong correlation between BMI and osteogenesis. Values reported as N ± SE; * p = 0.017.
Frazier et al. BMC Cell Biology 2013 14:34 doi:10.1186/1471-2121-14-34