Open Access Research article

ERK-associated changes in E2F4 phosphorylation, localization and transcriptional activity during mitogenic stimulation in human intestinal epithelial crypt cells

Marie-Christine Paquin, Sébastien Cagnol, Julie C Carrier, Caroline Leblanc and Nathalie Rivard*

Author Affiliations

Département d’Anatomie et Biologie Cellulaire, Cancer Research Pavillon, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, 3201, Jean-Mignault, Sherbrooke, J1E4K8, QC, Canada

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BMC Cell Biology 2013, 14:33  doi:10.1186/1471-2121-14-33

Published: 6 August 2013

Additional files

Additional file 1: Figure S1:

Subconfluent HIEC were serum-deprived for 36 h, treated or not (DMSO) during 10 min with the MEK inhibitors U0126 (20 μM) or PD184352 (5 μM) and then stimulated with 100 ng/ml EGF for 30 min. Thereafter, cells were lysed and proteins were analyzed by SDS-PAGE for Western blot analysis for the expression of phosphorylated ERK5, phosphorylated AKT, total AKT and β-actin.

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Additional file 2: Figure S2:

Subconfluent HIEC were serum-deprived for 36 h, treated or not (DMSO) during 10 min with the MEK inhibitors U0126 (20 μM) or PD184352 (5 μM) and then stimulated with 10% serum for 30 min. Thereafter, cells were lysed and proteins were analyzed by SDS-PAGE for Western blot analysis for the expression of E2F4, ERK2, phosphorylated ERK2 and β-actin. Representative of three experiments.

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